Quadrupole Time Of Flight – Mass spectrometry

Bruker MaXis II QTOF-MS coupled with ETD (Electron Transfer Dissociation) fragmentation offers distinct advantage of high resolution capabilities along with enhanced fragmentation techniques. It offers high resolution of 80000 and a mass error of 5 ppm, 5 orders of magnitude and dynamic range achieved at UHPLC speeds due to 50 GBit/s sampling technology. ETD involves the electron transfer from flouranthene ions to the protein of interest. The transferred electron randomly dissects the N-Cα amide bond, in-between the amino acid residue producing ladder series and giving the protein sequence information.

Model

Bruker MaXis II ETD, HRMS

Assays

  • Antibody characterization studies: Intact mass, light chain and Heavy chain analysis
    Antibody Drug Conjugate (ADC) and Drug Antibody Ratio (DAR) analysis
    Host Cell Protein studies for assessing process specific impurities in Biopharmaceuticals
    Peptide mapping studies, N/C-Terminal sequencing, Di-sulphide mapping
    Site specific glycosylation and Post Translational Modifications (PTMs) studies, Lysine truncation, proline amidation, Critical Quality Attributes (CQA) monitoring.

Resources

Resources

The following application notes are available for download in pdf format: