HPLC – High Performance Liquid Chromatography, UPLC

High-performance liquid chromatography (HPLC) or Ultra-performance liquid chromatography is an analytical technique widely used for separation, identification and quantification of various biological analytes. It relies on high pressure pumps to transfer a solvent through a packed column and separate the molecules based on its interaction with solvents and packed column material. The detection can be achieved depending on the spectral characteristics of the analyte. Various detectors including RI, PDA, UV, CAD,ELSD, MALS and FLD are available for orthogonal analysis, a suitable detector need to be selected based on the specific application.

Model

Agilent 1260 – 1290, Shimadzu Nexera – iSeries, Thermo Vanquish, Waters Acquity

Assays

  • Charge variants and heterogeneity analysis of complex large molecules using IEX chromatography
    Glycan quantification using FLD detector on UPLC/HPLC
    Non-volatile excipient detection and quantification using CAD HPLC
    Peptide mapping analysis using UPLC/HPLC
    Protein quantification using HPLC/UPLC
    SEC for purity assessment of protein and large molecules

Resources